View AbstractPlants are a source of large number of secondary metabolites comprising to different groups such as alkaloids, terpenoids, flavonoids, steroid, tannins, etc. They can act as antibacterial, antioxidant, antiulcer, anti-inflammatory, antiviral and anticancer agents. It is now believed that nature has given the cure of every disease in one way or another. Therefore, the present study was taken conduct the preliminary phytochemical screening, total flavonoid and phenolic contents assays of various leaf extracts (Hexane, methanolic and aqueous) of Juniperus squamata. Total flavonoid content was measured by the aluminium chloride colorimetric assay and total phenolic content was estimated spectrophotometrically by Folin-Ciocalteau method. Preliminary phytochemical screening reveals the presence of phenolics, flavonoids, alkaloids, terpenoids, phylobatanins and cardiac glycosides in methanolic and aqueous extracts.While hexane extract was found to contain steroids mainly. Moreover, in all the three different extracts (hexane ,methanolic, and aqueous) highest content of both phenolics and flavonoids was found in the methanolic extract i.e. 427 mg GAE/g and 255 mg RU/g respectively, and hexane extract with the least i.e. 132 mg GAE/g and 64 mg RU/g.